Globe rupture with aphakia, aniridia, secondary glaucoma and late corneal decompensation: A multi-staged approach to management

Severe blunt ocular trauma may result in immediate and delayed complications requiring appropriate management algorithms. We hereby report a case of globe rupture, aphakia, traumatic aniridia, and secondary glaucoma in a 33-year-old male following road traffic accident. He was treated initially by primary repair followed by novel combined approach of aniridia IOL with Ahmed glaucoma valve implantation. Delayed corneal decompensation required deferred penetrating keratoplasty. After a follow-up of 3.5 years after last surgery, patient maintains good functional vision with stable IOL, clear corneal graft and controlled intraocular pressure. A meticulously planned and staged management approach appears better suited in complex ocular trauma in such scenarios giving a good structural and functional outcome

Impact of mannitol and poly ethylene glycol 6000 induced water deficit on plant biomass and major secondary metabolites in Centella asiatica (L.) Urb. in vitr

Low water potential related stresses are regulated by modifying water uptake and loss to avoid low water potential, accumulating solutes which in turn enhance active principles and its gene expressions. Present study examined effect of in vitro induced absorption of mannitol and PEG (poly ethelene glycol) 6000 in Indian pennywort, Centella asiatica (L.) Urb., neutraceutical plant, evidenced by phenotypic, molecular and phytochemical analyses. Both mannitol and PEG 6000 induce water deficit conditions in plants and retarded normal plant biomass in terms of fresh and dry weights. These effects were significantly less severe in plants subjected to mannitol, compared to PEG. PEG and mannitol imposed water deficit, resulted in decline in major active compound, asiaticoside evidenced by HPTLC of asiaticoside content. Differential expression of some selected key genes in the asiaticoside pathway including squalene synthase and ? amyrin synthase by qPCR, confirmed decrease in transcript level expression of asiaticoside, whereas upregulated transcript level expression was observed in cycloartenol synthase for synthesis of phytosterols. Estimation of total flavonoids and phenolics under different water deficit conditions were found declined. In conclusion, water deficit by mannitol and PEG 6000 can significantly affects processes associated with biomass growth and ability to synthesize secondary metabolites in C. Asiatica.

Effect of dimethylaminoethanol intradermal injection on collagen synthesis in an aging model of rats / 中华医学美学美容杂志

Objective:To evaluate the effects of cellulift ? administered intradermally by mesotherapy on collagen synthesis in D-galactose induced aging model of rats. Methods:The study was conducted between April and October in 2014 in the Department of Anatomy, Qindao University. 30 male rats were randomly allocated to three groups: aging treatment group, aging control group and normal group; each group had ten rats. Aging treatment group and the control group were subcutaneously injected with D-galactose prepared in saline 125 mg·kg -1·d -1 for 42 day. Normal group was injected with saline for 42 d with same method and dose. From the 18th day after shaving their hair, the dermis of two sides hip skin marked zone of aging treatment group were injected cellulift at a dose of 1 ml per week for 4 weeks. Meanwhile, the aging control group was administrated the same volume of saline with same method. In vivo skin collagen alterations were investigated by reflectance confocal microscopy 3 days after every treatment. Skin specimens were obtained in 42 days. In order to measure the dermal collagen density and dermal thickness, HE and Masson trichrome staining were performed, respectively. Immunohistochemical staining for TGFβ1 and proliferating cell nuclear antigen (PCNA) was performed. Also, the level of TGFβ1, Smad3, types Ⅰ and Ⅲ pro-collagen mRNA expression was assessed by real-time quantitative polymerase chain reaction. Results:As revealed by RCM, collagen density of aging treatment group increased gradually after treatments, while in aging control group it decreased with time. Measurement of dermal thickness, hydroxyproline content and TGFβ-1 mRNA and protein expression in treatment group increased significantly as compared with that in aging control group, but were significantly lower than that in normal group (F values were 25.45, 98.90, 37.94 and 21.35, respectively; P<0.05). Measurement of dermal collagen density, the mRNA expression of type I pre-collagen and Smad3 elevated over that of aging control group with significant difference (F values were 44.46, 29.54 and 10.01, respectively; P<0.05), and there was no difference between normal and aging treatment group ( P>0.05). The difference of PCNA expression between aging control and treatment groups was not significant ( P>0.05), and both were lower than normal group ( P<0.05) . Conclusions:Cellulift ? shows anti-aging effects by activating collagen synthesis and eventually causing dermal thickening. This effect is probably mediated by TGF-β1/Smad3 signaling pathway.

Establishment of a gp120 transgenic mouse model with 7 nAChR knockout / 南方医科大学学报

OBJECTIVE@#To construct a HIV-1 gp120 transgenic mouse model (gp120) with 7 nicotinic acetylcholine receptor (7nAChR) gene knockout.@*METHODS@#The 7nAChR gene knockout mice (7R) were crossed with HIV-1gp120 transgenic mice (gp120) to generate F1 generation mice. We selected the F1 mice with the genotype of 7R/gp120 to mate to obtain the F2 mice. The genotypes of the F3 mice were identified by PCR, and the protein expressions in the double transgenic animal model was analyzed by immunohistochemistry. BV2 cells were treated with gp120 protein and 7nAChR inhibitor, and the expressions of IL-1β and TNF- were detected using ELISA.@*RESULTS@#The results of PCR showed the bands of the expected size in F3 mice. Two F3 mice with successful double gene editing (7R/gp120) were obtained, and immunohistochemistry showed that the brain tissue of the mice did not express 7 nAChR but with high gp120 protein expression. In the cell experiment, treatment with gp120 promoted the secretion of IL-1β and TNF- in BV2 cells, while inhibition of 7nAChR significantly decreased the expression of IL-1β and TNF- ( < 0.001).@*CONCLUSIONS@#By mating gp120 Tg mice with 7R mice, we obtained gp120 transgenic mice with 7nAChR gene deletion, which serve as a new animal model for exploring the role of 7nAChR in gp120-induced neurotoxicity.

Strong inflammation is essential for expression of articular cartilage-specific citrullinated antigens / 南方医科大学学报

OBJECTIVE@#To investigate the expression of citrullinated epitopes in articular cartilage protein and whether its expression is sufficient to induce anti-citrullinated protein antibody (ACPA) response in mice.@*METHODS@#The experimental group was treated with different concentrations of lipopolysaccharide (LPS), heat-inactivated bacteria ( and ) or specific monoclonal antibody against type Ⅱ collagen to induce citrullination of articular cartilage protein, with PBS as the control. Immunohistochemistry with the monoclonal antibody ACC4 (IgG1) that specifically binds to the citrullinated epitope of cartilage protein was performed for detecting the expression of citrullinated protein, with ACC1 (IgG2a) as a positive control antibody and L243 (IgG2a) and Hy2.15 (IgG1) as the negative isotype control. In the in vivo experiment, SD rats were subjected to injection of different doses of LPS in the right knee (with PBS as the controls in the left knee), and 3 days later frozen sections were prepared for immunohistochemical detection of the expression of citrullinated protein. Models of collagen-induced arthritis (CIA) established in different mouse strains were observed for incidence and severity of CIA. Serum samples collected from these models and the sera from rheumatoid arthritis patients were examined for anti-citrullinated protein antibody, and immunohistochemistry was performed to detect the expression of citrullinated protein in the cartilage of the mouse.@*RESULTS@#The citrullinated CII epitope-specific antibody ACC4 did not bind to articular cartilage tissues with different treatments as compared with the positive control antibody ACC1. The ACC4 antibody and the antibodies from patients with rheumatoid arthritis with high titers of anti-citrullinated protein antibody were capable of binding to the synovial tissue around the articular cartilage of the CIA. Luminex analysis showed that the anti-citrullinated protein antibody was lowly expressed in mouse serum, but the anti-type Ⅱ collagen triple helix structure peptide antibody exhibited strong reactivity.@*CONCLUSIONS@#Mild acute inflammatory response is not enough to cause citrullination of articular cartilage protein, and the expression of specific epitope requires a high-intensity inflammatory response. Inflammatory articular cartilage protein can express citrullinated epitopes in type Ⅱ collagen-induced arthritis in mice, but the expression of citrullinated epitopes is not sufficient to induce an immune response to anti-citrullinated antibodies. Stronger stimulation signals are required to induce an immune response for producing anti-citrullinated protein antibodies.

Relationship between fitness intervention and target organ damage in aged patients with essential hyper— tension / 心血管康复医学杂志

Objective :To analyze influence of fitness intervention on blood pressure control and prognosis in aged pa—tients with essential hypertension (EH).Methods : A total of 240 EH patients treated in our hospital were randomly and equally divided into routine treatment group and fitness intervention group .Blood pressure , quality of life (QOL) and incidence and development of target organ damage were compared between two groups after one month . Results : Compared with routine treatment group after one—month intervention ,there were significant reductions in blood pressure [(145. 65 ± 7. 89)／(98. 45 ± 9. 17) mmHg vs.(130.34 ± 8.98)／(82.11 ± 6.43) mmHg] ,scores of material life [ (78. 44 ± 8.23) scores vs.(64.68 ± 6.76) scores] ,mental function [ (76. 26 ± 7.54) scores vs.(67. 35 ± 6.65) scores] ,physical function [ (77.28 ± 7.53) scores vs.(59.28 ± 6. 12) scores] and social function [ (78. 73 ± 6.89) scores vs .(58.43 ± 6. 12) scores] of general quality of life inventory (GQOLI) ,carotid intima—media thick—ness [ (1. 05 ± 0.12) mm vs.(0.91 ± 0.09) mm] and brachial—ankle pulse wave velocity [ (1699.56 ± 240.90) mm／s vs .(1438. 56 ± 221. 78) mm／s] ,and significant rise in estimated glomerular filtration rate [ (63.21 ± 11.21) ml min－1 1.73m－2 vs .(76. 12 ± 10. 47) ml min－1 1. 73m－2 ] and ankle—brachial index [(0. 92 ± 0.13) vs.(1.00 ± 0.08)] in fitness intervention group , P＝ 0.001 all .Conclusion : Improving fitness can effectively control blood pressure , relieve degree of target organ damage and improve quality of life in aged patients with essential hypertension .

Application value of collective exercise motivation mode in patients with coronary heart disease / 心血管康复医学杂志

Objective :To explore influence of collective exercise motivation mode on quality of life (QOL) and prog-nosis in patients with coronary heart disease (CHD).Methods :A total of 116 CHD patients were randomly and e-qually divided into routine guidance group (received routine discharge guidance ) and exercise motivation group (re-ceived collective exercise motivation mode ).Seattle angina questionnaire (SAQ) was used to evaluate QOL ,thera-peutic compliance scale was used to assess therapeutic compliance ,and clinical prognosis was evaluated by major ad-verse cardiac events (MACE).Results :Compared with routine guidance group after intervention ,there were signif-icant rise in total SAQ score [ (52.4 ± 4.5) scores vs .(65.9 ± 5.4) scores] and therapeutic compliance (65.5% vs. 82.8%) ,and significant reduction in incidence rate of MACE (17.2% vs.5.2%) in exercise motivation group ,P＜0.05 or ＜0.01. Conclusion : Collective exercise motivation model can significantly improve quality of life ,thera-peutic compliance and prognosis in patients with coronary heart disease ,which is worth extending .

Genetic Polymorphism of 30 InDel Loci in Han Population from Jiangsu Province / 法医学杂志

Objective To investigate the genetic information of 30 insertion/deletion (InDel) loci in Han population from Jiangsu Province, and to explore the application values of Investigator? DIPplex kit for guiding the forensic analysis in Han population from Jiangsu Province. Methods The autosomal InDel loci of 305 unrelated healthy Han individuals from Jiangsu Province were genotyped and analysed by In-vestigator? DIPplex kit, and the allelic frequencies and forensic parameters of 30 InDel loci were statis-tically analysed. Results The distribution of 30 InDel loci in Han population from Jiangsu Province con-formed to Hardy-Weinberg equilibrium. The minor allele frequencies of 21 InDel loci were above 0.3. The polymorphism information content ranged from 0.089 to 0.375, while the discrimination power dis-tributed from 0.093 to 0.500. The paternity exclusion in duo cases and trio cases were 0.047-0.250 and 0.046-0.219, respectively. The linkage disequilibrium analysis of 30 InDel loci showed that all loci were independent from each other. The combined discrimination power was 1-7.369 ×10-8, whereas the com-bined mean exclusion chance in duo cases was 0.998933978, in trio cases was 0.997806392. The Fst values were all less than 0.06 except HLD118 and other four loci, which showed small differences be-tween groups. Conclusion The InDel loci of Investigator ? DIPplex kit can be used as complementary genetic markers for the cases associated with forensic genetics.

Early assessment of radiation-induced parotid damage with T2 ? mapping / 实用放射学杂志

Objective To monitor the dynamic changes of radiation-induced parotid damage using T2 ? mapping.Methods Thirty-four patients with nasopharyngeal carcinoma (NPC)were enrolled.All patients underwent T1 WI,T2 WI and T2 ? mapping for bilateral parotid glands at pre-RT (2 weeks before radiotherapy),mid-RT (5 weeks after the beginning of radiotherapy)and post-RT (4 weeks after the completion of radiotherapy).Parotid MR parameters,mean radiation dose and xerostomia degrees of the patients at different time points were recorded.Furthermore,nine healthy volunteers were enrolled,who undergone T2 ? mapping twice with an interval of 4 weeks in order to analyze the reproducibility of T2 ? value.Results From pre-RT to mid-RT and post-RT,parotid volume decreased [atrophy rates,(25.34±11.33)％ and (25.74±9.93)％,respectively]and T2 ? values decreased [change rates,(-5.63±8.86)％ and (-4.81±10.67)％, respectively]significantly (all P < 0.01 ).Parotid normalized T1 signal intensity decreased significantly from pre-RT to post-RT [change rate,(-7.43±10.61)％,P =0.007],and the change rate was correlated inversely with mean radiation dose significantly (r =-0.646, P <0.001).Parotid volume and T2 ? value changed correspondingly with xerostomia degrees of the patients during radiotherapy.Parotid MR parameters showed excellent reproducibility (intraclass correlation coefficient,0.843 -0.993).Conclusion The dynamic changes of radiation-induced parotid damage in patients with NPC can be noninvasively evaluated by routine MRI and T2 ? mapping.

Early assessment of radiation-induced parotid damage with T2 ? mapping / 实用放射学杂志

Objective To monitor the dynamic changes of radiation-induced parotid damage using T2 ? mapping.Methods Thirty-four patients with nasopharyngeal carcinoma (NPC)were enrolled.All patients underwent T1 WI,T2 WI and T2 ? mapping for bilateral parotid glands at pre-RT (2 weeks before radiotherapy),mid-RT (5 weeks after the beginning of radiotherapy)and post-RT (4 weeks after the completion of radiotherapy).Parotid MR parameters,mean radiation dose and xerostomia degrees of the patients at different time points were recorded.Furthermore,nine healthy volunteers were enrolled,who undergone T2 ? mapping twice with an interval of 4 weeks in order to analyze the reproducibility of T2 ? value.Results From pre-RT to mid-RT and post-RT,parotid volume decreased [atrophy rates,(25.34±11.33)％ and (25.74±9.93)％,respectively]and T2 ? values decreased [change rates,(-5.63±8.86)％ and (-4.81±10.67)％, respectively]significantly (all P < 0.01 ).Parotid normalized T1 signal intensity decreased significantly from pre-RT to post-RT [change rate,(-7.43±10.61)％,P =0.007],and the change rate was correlated inversely with mean radiation dose significantly (r =-0.646, P <0.001).Parotid volume and T2 ? value changed correspondingly with xerostomia degrees of the patients during radiotherapy.Parotid MR parameters showed excellent reproducibility (intraclass correlation coefficient,0.843 -0.993).Conclusion The dynamic changes of radiation-induced parotid damage in patients with NPC can be noninvasively evaluated by routine MRI and T2 ? mapping.

Mechanism of miR?21 in delayed ischemia preconditioning and its protection against subsequent ischemia reperfusion injury in kidney / 中华肾脏病杂志

Objective To investigate the molecular mechanism of protection of ischemia preconditioning on renal ischemia reperfusion injury. Methods Male C57/BL6N mice were randomly divided into two groups: in IR group, 35 min ischemia was induced by occlusion of both renal pedicles followed by 24 h perfusion (I/R). 15 min ischemia was induced 4 days before I/R in IPC group. Blood sample and kidney were collected in IR and IPC group after 24 h perfusion. Serum creatinine (Scr) and histological changes were used to evaluate the renal injury. PHD2 and HIF-1αwere evaluated by Western blotting, miR-21 expression was confirmed by real-time PCR. In vitro, hypoxic model was established by 1% O2 in HK-2 cells. Knockdown of miR-21 in hypoxic model was perfermed by locked nucleic acid modified-anti-miR-21 transfection. The levels of miR-21, HIF-1α and PHD2 mRNA were confirmed by real-time PCR. The levels of HIF-1α and PHD2 proteins were tested by Western blotting. Results In vivo, Compared with IR group, the renal function and histological changes were improved in IPC group (P<0.01). Compared with IR group, the expression of miR-21(P<0.01) and HIF-1α(P<0.05) were increased in IPC group, while PHD2 was reduced (P<0.01). In vitro, hypoxia reduced miR-21. The inhibition of miR-21 could increased the expression of PHD2 (P<0.05). Conclusions Ischemia preconditioning may exert protection against renal ischemia reperfusion injury by inhibiting PHD2.

Effect of miR-185 * on BDNF/TrkB signaling pathway expression in epileptic neurons / 西安交通大学学报(医学版)

ABSTRACT:Objective To study the BDNF/TrkB signaling pathway in the epilepsy model of hippocampal neurons and the regulatory effect of on it.Methods The primary hippocampal neurons cultured in vitro for 7 days were randomly divided into seven groups:control group,epilepsy group,control+BDNF group,epilepsy+BDNF group,control + miR-185 ? group,epilepsy + miR-185 ? group,and epilepsy + miR-185 ? + BDNF group.We constructed miR-185 ? lentivirus vector and observed the changes of BDNF/TrkB pathway expression after transfaction of miR-185 ? by immunohistochemistry,patch clamp technique and Western blot technique.Results Compared with the control+BDNF group,the phosphorylated TrkB (pTrkB)/TrkB value was significantly lower in epilepsy+BDNF group (P < 0.05 )and control group (P < 0.001 ).Compared with the epilepsy group,the phosphorylated TrkB (pTrkB)/TrkB value was significantly higher in epilepsy+BDNF group (P <0.05).Compared with the epilepsy+miR-185 ? +BDNF group,the phosphorylated TrkB (pTrkB)/TrkB value was significantly lower in epilepsy + BDNF group and epilepsy + miR-185 ? group (P < 0.001 ).BDNF could promote the signaling conduction and miR-185 ? could remove the inhibition of BDNF/TrkB signaling.Conclusion BDNF can activate the BDNF/TrkB signaling pathway and transfection with miR-185 ? can relieve the inhibition of BDNF/TrkB signaling pathway of epileptic state by up-regulating the expression of TrkB.

Par?metros de influ?ncia no ensaio de microtra??o de sistemas adesivos / Parameters that influence microtensile bond testing of adhesivesystems

The aim of this study was to provide a critical review of the literature regarding factors that can interfere with microtensile test results for enamel and dentin adhesive systems. Primarily, reports in English, Spanish, or Portuguese that were published between July 1994 and September 2009 and are catalogued in MEDLINE and BBO were used. Additionally, we compiled relevant articles found in the references of these articles anddissertations and theses available in electronic databases of Brazilian universities that examined factors that can influence implementationof the microtensile test at each stage. The search strategy included searching for the following key term groups: microtensile and test;microtensile and assay; microtensile and test and parameters; microtensile and test and factors; microtensile and specimen; microtracc?n andespecimen. We reviewed 25 selected articles and found that they showed that even after adjustment of test parameters, changes found in thedentin could be responsible for variations observed amongst results. This influence could potentially be reduced by using the cohesive strengthvalue of dentin adjacent to the adhesive interface as a standard for comparison, but more studies are needed to confirm whether such anapproach would be reliable. Data analysis methodology should be taken into account when comparing studies.

O objetivo deste estudo foi realizar uma revis?o cr?tica da literatura sobre os fatores que podem interferir nos resultados do teste de microtra??ode sistemas adesivos ao esmalte ou dentina. Foram utilizados trabalhos publicados nas bases de dados eletr?nicas MEDLINE e BBO, em ingl?s,espanhol ou portugu?s, entre julho de 1994 e setembro de 2009, refer?ncias destes artigos, al?m de disserta??es e teses dispon?veis em bancos de dados eletr?nicos de universidades brasileiras enfocando o estudo das vari?veis que podem influenciar cada fase da execu??o do ensaio de microtra??o. A estrat?gia de busca incluiu os termos: microtra??o e teste; microtra??o e ensaio; microtensile e test e parameters; microtensile e test e factors; microtensile e specimen; microtracci?n e esp?cimen. Os 25 artigos selecionados demonstraram que mesmo ap?s a padroniza??o de par?metros do ensaio, as altera??es encontradas na dentina podem ser respons?veis pela varia??o do resultado. Uma maneira de reduzir esta influ?ncia seria utilizar os valores de resist?ncia coesiva da dentina adjacente ? interface adesiva como padr?o de compara??o, por?m mais estudos s?o necess?rios. Aspectos relativos ? an?lise dos dados devem ser levados em considera??o quando se compara os estudos.

Tegumental histological effects of Mirazid? and myrrh volatile oil on adult Fasciola gigantica

To evaluated the histological changes within the tegument of adult Fasciola gigantica (F. gigantica) that led to the gross changes that were visible externally. Methods: The effects of oleoresin extract of myrrh (Mirazid ? ), myrrh volatile oil and triclabendazole sulphoxide (reference drug) on the tegumental structure of adult F. gigantica following treatment in vitro had been determined by light microscopy. Results: The internal changes in the tegument observed in this study were compatible with surface changes seen in the previous scanning electron microscopy study, using the same drugs. The swelling of tegumental syncytium was a particular feature of their action, but its level was much greater with myrrh volatile oil, in which vacuolization of the tegument and loss of spines were observed. Conclusions: The present study demonstrated the fasciocidal properties of Mirazid ? oleoresin extract, and it might be possible to reinforce its fasciocidal activity by increasing its content of myrrh volatile oil.

Algunas variables clínicas y de laboratorio en pacientes adultos con leucemia mieloide crónica tratados con interferón alfa recombinante + citosina arabinósido / Some clinical and laboratory variables in adult patients with chronic myeloid leukemia treated with recombinant alpha interferon + cytosine arabinoside

La leucemia mieloide crónica del adulto es la hemopatía maligna más frecuente dentro de los síndromes mieloproliferativos. En un estudio retrospectivo longitudinal realizado entre enero de 1985 y diciembre de 2009, se evaluaron 46 pacientes en fase crónica atendidos en el Instituto de Hematología e Inmunología. Todos recibieron tratamiento inicial citorreductor y posteriormente interferón ? recombinante (INF?r) + citosina arabinósido. El 41,0 por ciento de los enfermos presentó un índice pronóstico de Sokal de alto riesgo. Las manifestaciones clínicas más frecuentes al diagnóstico fueron astenia (37 por ciento), esplenomegalia (31 por ciento) y pérdida de peso (28,3 por ciento). La respuesta hematológica parcial y completa fue del 26,8 por ciento y 65,9 por ciento a los 6 meses; la respuesta citogenética y molecular completa de 9,1 por ciento y 16,3 por ciento, respectivamente. Las reacciones adversas más frecuentes fueron fiebre (34,9 por ciento), trombocitopenia (26,2 por ciento) y síndrome general (23,8 por ciento). El 47,8 por ciento de los casos mostraron resistencia o intolerancia al INF?r y el 90,9 por ciento falleció por progresión de la enfermedad. La sobrevida global a los 5 años fue del 63,8 por ciento y la sobrevida libre de eventos a los 3 años fue del 68,9 por ciento. Según el índice pronóstico de Sokal, la sobrevida global mostró diferencia significativa entre los 3 grupos (p= 0,005), no así para la sobrevida libre de eventos (p= 0,165). El tratamiento con INF?r mostró resultados superiores a los de algunos países desarrollados y constituye una opción terapéutica eficaz en Cuba

Chronic myeloid leukemia is the most frequent myeloproliferative syndrome in adults. In a longitudinal retrospective study performed between January 1985 - December 2009, 46 patients in chronic phase diagnosed at the Institute of Hematology and Immunology were evaluated. They received cytoreductor agent as first treatment followed by interferon ?2 + cytosar. Forty one percent showed high risk Sokal prognosis score. The most frequent clinical manifestations at diagnosis were asthenia (37 percent), splenomegaly (31 percent) and weigh lost (28.3 percent). The partial and complete hematological response was of 26,8 percent and 65.9 percent after 6 months and the complete cytogenetic and molecular response was of 9.1 percent and 16.3 percent. The most frequent adverse reactions were: fever (34.9 percent), thrombocytopenia (26.3 percent) and general syndrome (23.8 percent). Resistance or intolerance to INF?2 was found in 47.8 percent of the patients and 90.0 percent died due to progression of the disease. The 5 year overall survival was of 63.8 percent and the 3 years free event survival was of 68.9 percent. According to Sokal prognosis score the overall survival showed significant difference between groups (p= 0.005) but there was no significant difference for free event survival (p= 0.165). The INF?2 treatment in our patients showed better results than those obtained in different developed countries and is an effective therapeutic option in Cuba

LER/DORT: que métodos de avaliação do risco? / WRULMSDs: selection of risk assessment methods

O processo de seleção dos métodos observacionais de avaliação do risco de LER/DORT é complexo, mas fundamental para a gestão do risco. Em Setúbal, no período de 2004 a 2005, numa empresa portuguesa da indústria automobilística, onde a avaliação do risco foi previamente efetuada, reaplicou-se o método OCRA checklist (avaliação do risco de LER/DORT ao nível dos membros superiores) em postos de trabalho (n=152) montagem final e pintura classificados de risco moderado/elevado (OCRA ?16,5). Nas situações em que se reconfirmou a presença de risco (n=71), aplicaram-se três outros métodos: RULA, SI e HAL. Registraram-se sequências em vídeo da atividade de trabalho para avaliar a validade preditiva dos métodos. Revelam-se divergências entre os métodos na classificação dos postos de trabalho de risco elevado: OCRA 34 postos; HAL 35 postos; SI 31 postos; RULA 7 postos. A análise do efeito de cada variável independente (fator de risco) nos scores finais dos métodos (regressão linear multivariada) revela distintos contributos e processos de ponderação/avaliação do risco de LER/DORT. A análise dos registros em vídeo evidencia diversas validades preditivas relativamente aos fatores de risco integrantes dos métodos aplicados. Os resultados indicam a necessidade de uma seleção do método de avaliação do risco de LER/DORT adequada a cada situação (real) de trabalho, baseada no maior conhecimento dos métodos.

The process for selecting observational methods for evaluating work-related upper limb musucloskeletal disorders (WRULMSDs) is complex, but essential to risk management. In a Portuguese automotive industry plant (Setúbal, 2004-05), where all jobs had been previously analyzed, OCRA checklist was reapplied (final assembling and painting) in workstations (n=152) classified as moderate/ high risk (score 16.5 OCRA). In all risk confirmed cases (n=71) three other methods were applied: RULA, SI, and HAL. Sequences of working activities were recorded on video to estimate the predictive validity of the methods. The results show differences in the final scores of the methods in the same workstations: OCRA reveals 34 workplaces with high risk; SI only ranked 31 positions with high risk; HAL has 35 high risk workplaces, and RULA only 7. Analysis of the effect of each independent variable (risk factor) in the final scores of the methods, based on linear regression analysis, reveals different contributions and, consequently, different ways of assessing WRULMSD risk. The video analysis also highlights different predictive validities for the risk factors in each method. These results indicate the need to select the appropriate WRULMSD risk assessment instrument adequate to each real work situation.

Preliminary observation of the relationship between OGTT results in hyperthyroidism and pancreatic ? cell function / 中国糖尿病杂志

Objective To investigate the effect of pancreatic ? cell dysfunction on abnormal glucose tolerance during Graves hyperthyroidism. Methods We investigated 6 healthy people and 36 Graves disease patients with abnormal glucose tolerance, and determined blood sugar and insulin at 0, 60 and 120 minutes after breakfast respectively. Then the HOMA-IR, HOMA-? and ISI were compared between the two groups. Results The HOMA-IR in the Graves disease patients with abnormal glucose tolerance is evidently higher than in the healthy subjects (P